Direct AHG Test (DAT)

Testing products for Direct AHG Test (DAT)

LISS/Coombs

LISS/Coombs

The most important function of the polyspecific AHG reagent is to detect the presence of IgG. The importance of anti-complement in the AHG reagent is debatable since antibodies detectable only by their ability to bind complement are rather rare. However, anti-C3d activity is important for the DAT in the investigation of autoimmune hemolytic anemia (AIHA).

ID-Card (Id-n°: 50531):
•Available in a selection of package sizes
•Configuration: 6 x AHG (Anti-IgG + C3d) tests
•C3d: cell line C139-9

Ordering information

Pkg. size Profiles or single test REF
4 x 12 288 Tests 004014
24 x 12 1728 Tests 004017
60 x 12 4320 Tests 004016
112 x 12 8064 Tests 004015

Coombs Anti-IgG

Coombs Anti-IgG

The ID-Card Coombs Anti-IgG consists of 6 microtubes containing anti-human globulin anti-IgG (rabbit), within the gel matrix.

ID-Card (Id-n°: 50540):
•Configuration: 6 x AHG (Anti-IgG), rabbit

Ordering information

Pkg. size Profiles or single test REF
4 x 12 288 Tests 004024
24 x 12 1728 Tests 004027
60 x 12 4320 Tests 004026
112 x 12 8064 Tests 004025

DC-Screening II

DC-Screening II

A positive direct antiglobulin test (DAT) with polyspecific antihuman globulin (AHG) generally indicates that the red cells are coated in vivo with immunoglobulin and/or complement. To differentiate the reaction, monospecific AHG reagents are used, such as anti-IgG, -IgA, -IgM, -C3, -C3b, -C3c, -C3d and -C4.

ID-Card (Id-n°: 50560):
•Configuration: IgG, C3d, ctl/IgG, C3d, ctl
•IgG: rabbit, C3d: cell line C139-9

Ordering information

Pkg. size Profiles or single test REF
1 x 12 24 profiles 004831
24 x 12 576 profiles 004837
60 x 12 1440 profiles 004836

DC-Screening I

DC-Screening I

A positive direct antiglobulin test (DAT) with polyspecific antihuman globulin (AHG) generally indicates that the red cells are coated in vivo with immunoglobulin and/or complement. To differentiate the reaction, monospecific AHG reagents are used, such as anti-IgG, -IgA, -IgM, -C3, -C3b, -C3c, -C3d and -C4.

ID-Card (Id-n°: 50830):
•Configuration: IgG, IgA, IgM, C3c, C3d, ctl
•IgG: rabbit, IgA: rabbit, IgM: rabbit, C3c: rabbit,
C3d: cell line C139-9

Ordering information

Pkg. size Profiles or single test REF
1 x 12 12 profiles 004851
24 x 12 288 profiles 004857
60 x 12 720 profiles 004856

DAT IgG1/IgG3

DAT IgG1/IgG3

In the ID-Card DAT IgG1/IgG3, two dilutions of both anti-IgG1 and IgG3 are added to the gel to allow for the differentiation between low and high risk for hemolysis. A positive reaction with the first dilution has a sensitivity of approximately 1000 IgG1 and 125 anti-IgG3 molecules/cells, respectively. A positive reaction with the second dilution indicates a high concentration of anti-IgG1 and/or IgG3 antibodies. To further estimate the risk of hemolysis, the titration of IgG should be performed by using the ID-Card DAT IgG-Dilution.

ID-Card (Id-n°: 50890):
•IgG subclasses: IgG1: cell line M345/795, IgG3: cell line M346/805

Ordering information

Pkg. size Profiles or single test REF
1 x 12 12 profiles 004043

DAT IgG-Dilution

DAT IgG-Dilution

The numbers of IgG molecules per cell influence the accelerated red cell destruction seen in Autoimmune Hemolytic Anemia (AIHA), Hemolytic Disease of the Newborn (HDN) and transfusion reactions.

The ID-Card DAT IgG-Dilution provides an indication of the clinical importance of the DAT positive result.

ID-Card (Id-n°: 50870):
•Rabbit
•Configuration: 1:10, 1:30, 1:100, 1:300, 1:1000, ctl

Ordering information

Pkg. size Profiles or single test REF
1 x 12 12 profiles 004033