The most important function of the polyspecific AHG reagent is to detect the presence of IgG. The importance of anti-complement in the AHG reagent is debatable since antibodies detectable only by their ability to bind complement are rather rare. However, anti-C3d activity is important for the DAT in the investigation of autoimmune hemolytic anemia (AIHA).

ID-Card (Id-n°: 50531):
•Available in a selection of package sizes
•Configuration: 6 x AHG (Anti-IgG + C3d) tests
•C3d: cell line C139-9

The ID-Card Coombs Anti-IgG consists of 6 microtubes containing anti-human globulin anti-IgG (rabbit), within the gel matrix.

ID-Card (Id-n°: 50540):
•Configuration: 6 x AHG (Anti-IgG), rabbit

A positive direct antiglobulin test (DAT) with polyspecific antihuman globulin (AHG) generally indicates that the red cells are coated in vivo with immunoglobulin and/or complement. To differentiate the reaction, monospecific AHG reagents are used, such as anti-IgG, -IgA, -IgM, -C3, -C3b, -C3c, -C3d and -C4.

ID-Card (Id-n°: 50560):
•Configuration: IgG, C3d, ctl/IgG, C3d, ctl
•IgG: rabbit, C3d: cell line C139-9

A positive direct antiglobulin test (DAT) with polyspecific antihuman globulin (AHG) generally indicates that the red cells are coated in vivo with immunoglobulin and/or complement. To differentiate the reaction, monospecific AHG reagents are used, such as anti-IgG, -IgA, -IgM, -C3, -C3b, -C3c, -C3d and -C4.

ID-Card (Id-n°: 50830):
•Configuration: IgG, IgA, IgM, C3c, C3d, ctl
•IgG: rabbit, IgA: rabbit, IgM: rabbit, C3c: rabbit,
C3d: cell line C139-9

In the ID-Card DAT IgG1/IgG3, two dilutions of both anti-IgG1 and IgG3 are added to the gel to allow for the differentiation between low and high risk for hemolysis. A positive reaction with the first dilution has a sensitivity of approximately 1000 IgG1 and 125 anti-IgG3 molecules/cells, respectively. A positive reaction with the second dilution indicates a high concentration of anti-IgG1 and/or IgG3 antibodies. To further estimate the risk of hemolysis, the titration of IgG should be performed by using the ID-Card DAT IgG-Dilution.

ID-Card (Id-n°: 50890):
•IgG subclasses: IgG1: cell line M345/795, IgG3: cell line M346/805

The numbers of IgG molecules per cell influence the accelerated red cell destruction seen in Autoimmune Hemolytic Anemia (AIHA), Hemolytic Disease of the Newborn (HDN) and transfusion reactions.

The ID-Card DAT IgG-Dilution provides an indication of the clinical importance of the DAT positive result.

ID-Card (Id-n°: 50870):
•Rabbit
•Configuration: 1:10, 1:30, 1:100, 1:300, 1:1000, ctl